ski 1 inhibitor  (MedChemExpress)

Journal: Journal of Inflammation (London, England)

Article Title: Different roles for non-receptor tyrosine kinases in arachidonate release induced by zymosan and Staphylococcus aureus in macrophages

doi: 10.1186/1476-9255-3-8

Figure Lengend Snippet: Inhibition by PP2 or SKI-1 of zymosan- and S.aureus -induced release of arachidonate . Macrophages were labeled with [3H]arachidonic acid for 20 h. The cells were pretreated for 15 min with the indicated concentrations of PP2 (A) or SKI-1 (B) followed by stimulation with either zymosan (●) for 45 min or S.aureus (▲) for 60 min. Results are mean ± SEM (n = 3) and corrected for the release in control cultures.

Article Snippet: LFM-A13 and Src kinase inhibitor I (SKI-1) from Calbiochem (La Jolla, CA, USA).

Techniques: Inhibition, Labeling

Journal: Journal of Inflammation (London, England)

Article Title: Different roles for non-receptor tyrosine kinases in arachidonate release induced by zymosan and Staphylococcus aureus in macrophages

doi: 10.1186/1476-9255-3-8

Figure Lengend Snippet: Inhibition by PP2 or SKI-1 of zymosan- and S.aureus - induced phosphorylation of MAP kinases . Macrophages were pretreated for 15 min with PP2 (1–10 μM), followed by stimulation with zymosan ( A ) or S.aureus ( B ) for 20 min. ( C ) Macrophages were pretreated for 15 min with SKI-1 (5 μM), followed by stimulation with zymosan or S.aureus for 20 min. Equal amounts of cell lysate were run on 10% polyacrylamide gels and probed with phosphospecific antibodies against ERK, p38 and JNK. The membrane was reprobed with ERK-2 antibody to verify equal loading of protein.

Article Snippet: LFM-A13 and Src kinase inhibitor I (SKI-1) from Calbiochem (La Jolla, CA, USA).

Techniques: Inhibition

Journal: Journal of Inflammation (London, England)

Article Title: Different roles for non-receptor tyrosine kinases in arachidonate release induced by zymosan and Staphylococcus aureus in macrophages

doi: 10.1186/1476-9255-3-8

Figure Lengend Snippet: Zymosan but not S.aureus induced tyrosine phosphorylation of PLC γ2 . ( A ) Macrophages were stimulated with zymosan(z) or S.aureus ( S.a ) for 45 min. (B and C) Macrophages were pretreated for 15 min with either PP2 (5 μM), wortmannin (W, 100 nM) ( B ) or SKI-1 (5 μM) ( C ) followed by stimulation with zymosan for 30 min. Cell lysates were immunoprecipitated with antibody against PLCγ2 as described, followed by Western blot analysis with phosphotyrosine-specific antibody. The membrane was stripped and reprobed with antibody against PLCγ2. The data are representative of three separate experiments.

Article Snippet: LFM-A13 and Src kinase inhibitor I (SKI-1) from Calbiochem (La Jolla, CA, USA).

Techniques: Immunoprecipitation, Western Blot

Journal: Journal of Inflammation (London, England)

Article Title: Different roles for non-receptor tyrosine kinases in arachidonate release induced by zymosan and Staphylococcus aureus in macrophages

doi: 10.1186/1476-9255-3-8

Figure Lengend Snippet: Zymosan and bacteria but not LPS or peptidoglycan (PGN) induce phosphorylation of Btk . ( A ) Macrophages were stimulated with zymosan (zym), S.aureus ( S.a .), LPS or PGN for 30 min. (B and C) Macrophages were pretreated for 15 min with either wortmannin (W, 100 nM), PP2 (5 μM), SU6656 (5 μM) or SKI-1 (5 μM), followed by stimulation with zymosan ( B ) or S.aureus ( C ) for 30 min. Equal amounts of cell lysate were run on polyacrylamide gels and probed with phosphospecific antibodies against Btk. The membrane was reprobed with ERK-2 antibody to verify equal loading of proteins. The data are representative of three separate experiments.

Article Snippet: LFM-A13 and Src kinase inhibitor I (SKI-1) from Calbiochem (La Jolla, CA, USA).

Techniques:

Journal: Viruses

Article Title: How Do Enveloped Viruses Exploit the Secretory Proprotein Convertases to Regulate Infectivity and Spread?

doi: 10.3390/v13071229

Figure Lengend Snippet: SKI-1/S1P processing sites of various cellular substrates and those of enveloped viruses with emphasis on the sequences surrounding their surface glycoprotein cleavage sites, designated by an arrow. The bold and underlined residues at positions P4 and P2 emphasize the importance of these amino acids for protease recognition.

Article Snippet: A small molecule SKI-1/S1P inhibitor PF-429242 was developed by Pfizer [ , ] and tested as an antiviral targeting GP-C processing and productive infection of arenaviruses.

Techniques: Virus

Journal: Oncogenesis

Article Title: Squalene synthase promotes the invasion of lung cancer cells via the osteopontin/ERK pathway

doi: 10.1038/s41389-020-00262-2

Figure Lengend Snippet: a CL1-0 cells infected with the vector and SQS overexpression plasmid were subjected to western blot analyses of SQS, pSrc, Src, pERK, ERK, pAKT 473 , AKT and α-tubulin levels. b Comparison of pSrc, Src, pERK, ERK, pAKT 473 , and AKT levels between SQS knockdown A549 and CL1-5 cells and nonsilenced shRNA control cells. c Western blots showing the levels of pSrc, Src, pERK, ERK, pAKT 473 , AKT and α-tubulin in CL1-0/SQS cells after treatment with Src kinase inhibitor-1 (SKI, 10 μM). d Heatmap showing the protein levels of AKT, AKT_pS473, Src, and Src_pY416 and migration ability in the lung cancer cell panel. e Plot showing the correlations between the protein levels of AKT_pS473 protein/Src_pY416 protein and migration in the lung cancer cell panel. f CL1-0/SQS cells were treated with or without DMSO, Src inhibitor-1 (SKI) (10 μM), PD98059 (20 μM) and LY294002 (10 μM). Top panel, The total protein samples were subjected to western blot analyses of SQS and α-tubulin levels (cell extract, CE). Middle panel, MMP1 protein expression levels from condition medium (CM). Bottom panel, Invasion (open) and migration (filled) of CL1-0/SQS cells treated with DMSO, SKI, PD98059 and LY294002 compared to CL1-0/Vector cells. Data are presented as the means ± SDs. ** P < 0.01. g CL1-0/SQS cells were treated with or without MβCD (10 mM) for 30 min 37 °C. Western blot analyses of SQS, pSrc, pERK and pAKT and α-tubulin levels of CL1-0/SQS cells. h Effect of cholesterol replenishment on the phosphorylation of Src, ERK, and AKT in CL1-0 cells.

Article Snippet: Src Inhibitor-1 (SKI) (Cat. S2075, 10 μM), PD-98059 (a mitogen-activated protein (MAP) kinase inhibitor, Cat. P215, 20 μM) and cholesterol (Cat.C3045) were purchased from Sigma, St. Louis, MO, USA.

Techniques: Infection, Plasmid Preparation, Over Expression, Western Blot, shRNA, Migration, Expressing